- Abstract:
- The aims of the project were to use database programmes to align the turkey and pork mitochondrial cytochrome b genes and design and obtain universal primers. These will be used to amplify a region from DNA extracted from samples of raw turkey, chicken, beef and pork. To then sequence the amplicons to determine the exact DNA sequence for the mitochondrial cytochrome b gene derived from turkey, chicken, beef and pork meats available in the UK. To align turkey, chicken, beef, and pork DNA sequences and design primers and probes. To aptimise PCR (and extraction conditions) for all primers on turkey, chicken, beef and pork. To compare two real-time PCR systems used (Amplifluor and Taqman). To perform DNA analysis of canned samples.
- PubYear:
- 2003
- Keywords:
- FundingBody:
- Food Standards Agency (UK)
- Contractor:
- Eurofins Scientific
- ProjCode:
- Q01033
- Associated:
- Type:
- Research: Laboratory Based
- OpenAccess:
- Yes
- URL:
- http://webarchive.nationalarchives.gov.uk/20141103165934/http://www.foodbase.org.uk/results.php?f_report_id=260
- Comments:
- NA